In order to debug all the issues please share the info requested and disable windows defender. First we have to know that the backdoor works and then we’ll deal with AV evasion.
Yes, you can use other ports rahter than 80 or 8080, actually 80 is not a good idea if you have an apache server running.
Another virus scanner could be https://www.virscan.org/language/en/.
Let me know.